Limited genetic diversity among clones of red wine cultivar 'Carmenère' as revealed by microsatellite and AFLP markers

Authors

  • X. Moncada
  • P. Hinrichsen

Keywords:

clonal selection, Vitis, microsatellites, SSR, AFLP, genetic diversity

Abstract

'Carmenère' is a fine red wine cultivar (Vitis vinifera L.) that has spread, unrecorded from France to  other countries. It probably arrived in Chile before the Phylloxera crisis in Europe where it remained confused with Merlot and other red wine cultivars until the mid 1990s. In this study, genetic diversity among 26 accessions from Chile, France and Italy was analysed using microsatellite (SSR) and AFLP markers. Using 20 SSR markers, a “standard genotype” was established and three different haplotypes were found, presumably arising by a mutation at the VVMD7 and VMC5g7 loci. In the case of AFLP, using 11 primer combinations five groups were identified, with one main cluster of 22 accessions not differentiated. Combining both techniques it was possible to identify five out of the 26 accessions analysed. Together, these results suggest that 'Carmenère' exhibits a lower genetic diversity in comparison with other French red wine cultivars. This is a factor to consider when managing a clonal selection assay. Possible causes are discussed.

 

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Published

2015-04-14

Issue

Vol. 46 No. 4 (2007): Vitis

Section

Article

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