Enhanced expression of radish-specific proteins in a disomic rapeseed-radish chromosome addition line resistant to root-knot nematodes

Authors

  • Ping Yu Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, P. R. China
  • Shao Song Zhang Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, P. R. China
  • Chao Dong Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, P. R. China
  • Hong Mei Luo Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, P. R. China
  • Holger Budahn Julius Kühn-Institut, Institute for Breeding Research on Horticultural Crops, Germany

Keywords:

rapeseed, radish, root-knot nematodes, Meloidogyne incognita, Meloidogyne javanica, resistance

Abstract

Root-knot nematodes cause significant economic losses worldwide each year. Species with especially high relevance in the tropical and subtropical part of China are Meloidogyne incognita and M. javanica. The disomic rapeseed-radish chromosome addition line ee was shown in former experiments to be highly resistant against these southern root-knot nematodes. In this study, differen­tially expressed proteins in the roots of the addition line ee, its radish donor line and its rapeseed recipient line cv. Madora were screened by two-dimensional gel electrophoresis and Image Master 5.0 software. These proteins were identified by MALDI-TOF-TOF/MS. Three radish-specific proteins with an increased expression in rapeseed-radish addition line ee will be further analyzed for their potential role in nematode resistance.

DOI: 10.1399/JfK.2018.07.01, https://doi.org/10.1399/JfK.2018.07.01

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Published

2018-07-01

Issue

Vol. 70 No. 7 (2018)

Section

Original Article